In Vitro Gene Delivery to Hepatocytes with Galactosylated Polyethylenimine
Identifieur interne : 002887 ( Main/Exploration ); précédent : 002886; suivant : 002888In Vitro Gene Delivery to Hepatocytes with Galactosylated Polyethylenimine
Auteurs : Maria-Antonietta Zanta [France] ; Otmane Boussif [France] ; Abdennaji Adib [France] ; Jean-Paul Behr [France]Source :
- Bioconjugate Chemistry [ 1043-1802 ] ; 1997.
Abstract
A hepatocyte-directed vector has been developed; it includes several key features thought to favor in vivo gene delivery to the liver: electrostatically neutral particles which avoid nonspecific binding to other cells, to the extracellular matrix, and to complement proteins; asialoglycoprotein receptor-mediated endocytosis which may address the complexes to the perinuclear region; and polyethylenimine (PEI)-mediated endosome buffering and swelling as an escape mechanism to the cytoplasm. This system is based on a 5% galactose-bearing polyethylenimine (PEI−gal) polymer which is condensed with plasmid DNA to neutrality. Murine (BNL CL.2) and human (HepG2) hepatocyte-derived cell lines were transfected 104−105-fold more efficiently than murine fibroblasts (3T3), whether transfection was assessed globally (luciferase expression from the cell extract) or following histochemical staining (β-galactosidase). Under these conditions, over 50% of the hepatocytes were selectively transfected in the presence of 10% serum. Transfection was suppressed by removal of the targeting galactose residues, by their replacement with glucose, or by the addition of excess asialofetuin. Thus, results from comparative and competitive experiments indicate the asialoglycoprotein receptor is involved in transfection of hepatocytes with neutral PEI−gal/DNA complexes.
Url:
DOI: 10.1021/bc970098f
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 001524
- to stream Istex, to step Curation: 001524
- to stream Istex, to step Checkpoint: 001680
- to stream Main, to step Merge: 002936
- to stream Main, to step Curation: 002887
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>In Vitro Gene Delivery to Hepatocytes with Galactosylated Polyethylenimine</title><author><name sortKey="Zanta, Maria Antonietta" sort="Zanta, Maria Antonietta" uniqKey="Zanta M" first="Maria-Antonietta" last="Zanta">Maria-Antonietta Zanta</name></author><author><name sortKey="Boussif, Otmane" sort="Boussif, Otmane" uniqKey="Boussif O" first="Otmane" last="Boussif">Otmane Boussif</name></author><author><name sortKey="Adib, Abdennaji" sort="Adib, Abdennaji" uniqKey="Adib A" first="Abdennaji" last="Adib">Abdennaji Adib</name></author><author><name sortKey="Behr, Jean Paul" sort="Behr, Jean Paul" uniqKey="Behr J" first="Jean-Paul" last="Behr">Jean-Paul Behr</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:6DC5FF21AECD81F838608C3CC0B3392FD7EA499E</idno><date when="1997" year="1997">1997</date><idno type="doi">10.1021/bc970098f</idno><idno type="url">https://api.istex.fr/ark:/67375/TPS-4SCTDBQS-X/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">001524</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001524</idno><idno type="wicri:Area/Istex/Curation">001524</idno><idno type="wicri:Area/Istex/Checkpoint">001680</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001680</idno><idno type="wicri:doubleKey">1043-1802:1997:Zanta M:in:vitro:gene</idno><idno type="wicri:Area/Main/Merge">002936</idno><idno type="wicri:Area/Main/Curation">002887</idno><idno type="wicri:Area/Main/Exploration">002887</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en"><hi rend="italic">In Vitro </hi>Gene Delivery to Hepatocytes with Galactosylated
Polyethylenimine</title><author><name sortKey="Zanta, Maria Antonietta" sort="Zanta, Maria Antonietta" uniqKey="Zanta M" first="Maria-Antonietta" last="Zanta">Maria-Antonietta Zanta</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Chimie Génétique associé au CNRS (URA 1386), Faculté de Pharmacie, Université Louis Pasteurde Strasbourg, BP24, F-67401 Illkirch Cedex</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Alsace (région administrative)</region><settlement type="city">Illkirch</settlement></placeName></affiliation></author><author><name sortKey="Boussif, Otmane" sort="Boussif, Otmane" uniqKey="Boussif O" first="Otmane" last="Boussif">Otmane Boussif</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Chimie Génétique associé au CNRS (URA 1386), Faculté de Pharmacie, Université Louis Pasteurde Strasbourg, BP24, F-67401 Illkirch Cedex</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Alsace (région administrative)</region><settlement type="city">Illkirch</settlement></placeName></affiliation></author><author><name sortKey="Adib, Abdennaji" sort="Adib, Abdennaji" uniqKey="Adib A" first="Abdennaji" last="Adib">Abdennaji Adib</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Chimie Génétique associé au CNRS (URA 1386), Faculté de Pharmacie, Université Louis Pasteurde Strasbourg, BP24, F-67401 Illkirch Cedex</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Alsace (région administrative)</region><settlement type="city">Illkirch</settlement></placeName></affiliation></author><author><name sortKey="Behr, Jean Paul" sort="Behr, Jean Paul" uniqKey="Behr J" first="Jean-Paul" last="Behr">Jean-Paul Behr</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Chimie Génétique associé au CNRS (URA 1386), Faculté de Pharmacie, Université Louis Pasteurde Strasbourg, BP24, F-67401 Illkirch Cedex</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Alsace (région administrative)</region><settlement type="city">Illkirch</settlement></placeName></affiliation><affiliation wicri:level="3"><country wicri:rule="url">France</country><wicri:regionArea> Address correspondence to J.-P. Behr, ChimieGénétique,Faculté de Pharmacie, BP 24, 67401 Illkirch Cedex</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Alsace (région administrative)</region><settlement type="city">Illkirch</settlement></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Bioconjugate Chemistry</title><title level="j" type="abbrev">Bioconjugate Chem.</title><idno type="ISSN">1043-1802</idno><idno type="eISSN">1520-4812</idno><imprint><publisher>American Chemical Society</publisher><date type="e-published" when="1997-11-26">1997</date><date when="1997-11-26">1997</date><biblScope unit="vol">8</biblScope><biblScope unit="issue">6</biblScope><biblScope unit="page" from="839">839</biblScope><biblScope unit="page" to="844">844</biblScope></imprint><idno type="ISSN">1043-1802</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1043-1802</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract">A hepatocyte-directed vector has been developed; it includes several key features thought to favor in vivo gene delivery to the liver: electrostatically neutral particles which avoid nonspecific binding to other cells, to the extracellular matrix, and to complement proteins; asialoglycoprotein receptor-mediated endocytosis which may address the complexes to the perinuclear region; and polyethylenimine (PEI)-mediated endosome buffering and swelling as an escape mechanism to the cytoplasm. This system is based on a 5% galactose-bearing polyethylenimine (PEI−gal) polymer which is condensed with plasmid DNA to neutrality. Murine (BNL CL.2) and human (HepG2) hepatocyte-derived cell lines were transfected 104−105-fold more efficiently than murine fibroblasts (3T3), whether transfection was assessed globally (luciferase expression from the cell extract) or following histochemical staining (β-galactosidase). Under these conditions, over 50% of the hepatocytes were selectively transfected in the presence of 10% serum. Transfection was suppressed by removal of the targeting galactose residues, by their replacement with glucose, or by the addition of excess asialofetuin. Thus, results from comparative and competitive experiments indicate the asialoglycoprotein receptor is involved in transfection of hepatocytes with neutral PEI−gal/DNA complexes.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Alsace (région administrative)</li><li>Grand Est</li></region><settlement><li>Illkirch</li></settlement></list><tree><country name="France"><region name="Grand Est"><name sortKey="Zanta, Maria Antonietta" sort="Zanta, Maria Antonietta" uniqKey="Zanta M" first="Maria-Antonietta" last="Zanta">Maria-Antonietta Zanta</name></region><name sortKey="Adib, Abdennaji" sort="Adib, Abdennaji" uniqKey="Adib A" first="Abdennaji" last="Adib">Abdennaji Adib</name><name sortKey="Behr, Jean Paul" sort="Behr, Jean Paul" uniqKey="Behr J" first="Jean-Paul" last="Behr">Jean-Paul Behr</name><name sortKey="Behr, Jean Paul" sort="Behr, Jean Paul" uniqKey="Behr J" first="Jean-Paul" last="Behr">Jean-Paul Behr</name><name sortKey="Boussif, Otmane" sort="Boussif, Otmane" uniqKey="Boussif O" first="Otmane" last="Boussif">Otmane Boussif</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/ChloroquineV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002887 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002887 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= ChloroquineV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:6DC5FF21AECD81F838608C3CC0B3392FD7EA499E
|texte= In Vitro Gene Delivery to Hepatocytes with Galactosylated Polyethylenimine
}}
| This area was generated with Dilib version V0.6.33. |  |